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Current testing for Lyme disease, called the standard 2-tiered approach or the STT, involves running two complex assays (ELISA and western blot) to detect antibodies against the bacterium, and requires experienced personnel in a lab, and a few hours to carry out and interpret. Biomedical engineers have developed a rapid microfluidic test that can detect Lyme disease with similar performance as the STT in a much shorter time — 15 minutes.